Alkaline Phosphatase Kit ELISA

N° du produit ABIN2702814

Détail du produit Alkaline Phosphatase Kit ELISA

Antigène: Alkaline Phosphatase (ALP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2-500 MioU/mL

Seuil minimal de détection: 2 MioU/mL

Application: ELISA

Fonction: Human Alkaline Phosphatase (ALPL) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human ALKP. Other species not determined.

Sensibilité: 2 mIU/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase (ALP Produits)

Synonymes: ALP Kit ELISA, ALP1 Kit ELISA, CRASH Kit ELISA, ECK0378 Kit ELISA, JW0374 Kit ELISA, psiA Kit ELISA, 2410004D18Rik Kit ELISA, AU040643 Kit ELISA, AW060375 Kit ELISA, ILC Kit ELISA, CTACK Kit ELISA, ESkine Kit ELISA, Akp2 Kit ELISA, alp Kit ELISA, zgc:56672 Kit ELISA, DDBDRAFT_0205491 Kit ELISA, DDBDRAFT_0231570 Kit ELISA, DDB_0205491 Kit ELISA, DDB_0231570 Kit ELISA, Actn2lp Kit ELISA, Alp Kit ELISA, asparaginase like 1 Kit ELISA, bacterial alkaline phosphatase Kit ELISA, C-C motif chemokine ligand 27 Kit ELISA, alkaline phosphatase, liver/bone/kidney Kit ELISA, alkaline phosphatase Kit ELISA, PDZ and LIM domain 3 Kit ELISA, ASRGL1 Kit ELISA, phoA Kit ELISA, Asrgl1 Kit ELISA, CCL27 Kit ELISA, alpl Kit ELISA, alp Kit ELISA, Pdlim3 Kit ELISA

Sujet: Gene Names: ALPL
Protein names: Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme)

ID gène: 249

UniProt: P05186