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Cathepsin L Kit ELISA

CTSL1 Reactivité: Humain Colorimetric Sandwich ELISA 24-6.000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702883
  • Antigène Voir toutes Cathepsin L (CTSL1) Kits ELISA
    Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
    Reactivité
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    24-6.000 pg/mL
    Seuil minimal de détection
    24 pg/mL
    Application
    ELISA
    Fonction
    Human Cathepsin L ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 2B4, ADAM-9, ANG-2, APRIL, BMP-2, BMP-9, C5a, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGF R, IFN-alpha/beta R2, IGF-II, IGF-IIR, IL-1 R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PD-1, PGRP-5, Serpin A4, sFRP-3, Thrombomodulin, TLR2, TRAIL R1, Transferrin, WIF-1.
    Sensibilité
    24 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Cathepsin L (CTSL1) Kits ELISA
    Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
    Autre désignation
    Cathepsin L (CTSL1 Produits)
    Synonymes
    CATL Kit ELISA, CTSL1 Kit ELISA, MEP Kit ELISA, 1190035F06Rik Kit ELISA, Ctsl1 Kit ELISA, fs Kit ELISA, nkt Kit ELISA, CATHL Kit ELISA, CatL Kit ELISA, Ctsl Kit ELISA, CTSL Kit ELISA, mep Kit ELISA, catl Kit ELISA, ctsl Kit ELISA, cpl-1 Kit ELISA, MGC80629 Kit ELISA, MGC107932 Kit ELISA, LOC663234 Kit ELISA, 26-29-p Kit ELISA, Cp1 Kit ELISA, si:dkey-121a11.6 Kit ELISA, CTSV Kit ELISA, catL Kit ELISA, cb15 Kit ELISA, ctsl1b Kit ELISA, hgg1 Kit ELISA, wu:fb30g09 Kit ELISA, zgc:92089 Kit ELISA, cathepsin L Kit ELISA, cathepsin V Kit ELISA, cathepsin L L homeolog Kit ELISA, cathepsin L1 Kit ELISA, Cathepsin L1 Kit ELISA, Cathepsin L Kit ELISA, testin gene Kit ELISA, cathepsin 12 Kit ELISA, cathepsin Lb Kit ELISA, cathepsin L.1 Kit ELISA, CTSL Kit ELISA, Ctsl Kit ELISA, CTSV Kit ELISA, ctsl.L Kit ELISA, ctsl Kit ELISA, LOC100008830 Kit ELISA, catl1 Kit ELISA, 26-29-p Kit ELISA, catl Kit ELISA, Tsp_11596 Kit ELISA, Testin Kit ELISA, cts12 Kit ELISA, LOC102156614 Kit ELISA, ctslb Kit ELISA, ctsl.1 Kit ELISA
    Sujet
    Gene Names: CTSL CTSL1
    Protein names: Cathepsin L1 (EC 3.4.22.15) (Cathepsin L) (Major excreted protein) (MEP) [Cleaved into: Cathepsin L1 heavy chain, Cathepsin L1 light chain]
    ID gène
    1514
    UniProt
    P07711
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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