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Tissue factor Kit ELISA
F3
Reactivité: Souris
Colorimetric
Sandwich ELISA
1.6-400 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702922
Détail du produit Tissue factor Kit ELISA
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Antigène
Voir toutes Tissue factor (F3) Kits ELISA
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Reactivité
Toutes les réactivités sur Tissue factor Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
1.6-400 ng/mL
Seuil minimal de détection
1.6 ng/mL
Application
ELISA
Fonction
Mouse Coagulation Factor III (Tissue Factor/Thromboplastin) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Mouse Coagulation Factor III (F3 / Tissue Factor / Thromboplastin)
Sensibilité
1.6 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Tissue factor (F3) Kits ELISA
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Autre désignation
Coagulation Factor III (F3 Produits )
Synonymes
CD142 Kit ELISA, TF Kit ELISA, TFA Kit ELISA, PRO1557 Kit ELISA, PRO2086 Kit ELISA, TFQTL1 Kit ELISA, f3 Kit ELISA, AA409063 Kit ELISA, Cf-3 Kit ELISA, Cf3 Kit ELISA, tf Kit ELISA, zgc:112151 Kit ELISA, coagulation factor III, tissue factor Kit ELISA, transferrin Kit ELISA, coagulation factor IIIa Kit ELISA, coagulation factor III Kit ELISA, tissue factor Kit ELISA, coagulation factor IIIb Kit ELISA, coagulation factor III (thromboplastin, tissue factor) S homeolog Kit ELISA, F3 Kit ELISA, TF Kit ELISA, f3a Kit ELISA, tf Kit ELISA, f3b Kit ELISA, f3.S Kit ELISA
Sujet
Gene Names: F3 Cf-3 Cf3 Protein names: Tissue factor (TF) (Coagulation factor III) (CD antigen CD142)
ID gène
14066
UniProt
P20352
Pathways
Positive Regulation of Endopeptidase Activity , Smooth Muscle Cell Migration , Platelet-derived growth Factor Receptor Signaling
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