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CSF3R Kit ELISA
CSF3R
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.1-25 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703035
Détail du produit CSF3R Kit ELISA
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Antigène
Voir toutes CSF3R Kits ELISA
CSF3R
(Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))
Reactivité
Toutes les réactivités sur CSF3R Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.1-25 ng/mL
Seuil minimal de détection
0.1 ng/mL
Application
ELISA
Fonction
Human GCSF R (CD114) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes human G-CSF R/CD114.
Sensibilité
0.1 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CSF3R Kits ELISA
CSF3R
(Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))
Autre désignation
G-CSF R (CSF3R Produits )
Synonymes
CD114 Kit ELISA, GCSFR Kit ELISA, Cd114 Kit ELISA, Csfgr Kit ELISA, G-CSF-R Kit ELISA, G-CSFR Kit ELISA, CSF3R Kit ELISA, gcsfr Kit ELISA, cd114 Kit ELISA, colony stimulating factor 3 receptor Kit ELISA, colony stimulating factor 3 receptor (granulocyte) Kit ELISA, colony stimulating factor 3 receptor L homeolog Kit ELISA, granulocyte colony-stimulating factor receptor Kit ELISA, CSF3R Kit ELISA, Csf3r Kit ELISA, csf3r Kit ELISA, csf3r.L Kit ELISA, LOC100433338 Kit ELISA
Sujet
Gene Names: CSF3R GCSFR Protein names: Granulocyte colony-stimulating factor receptor (G-CSF receptor) (G-CSF-R) (CD antigen CD114)
ID gène
1441
UniProt
Q99062
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