Ghrelin Kit ELISA

N° du produit ABIN2703051

Détail du produit Ghrelin Kit ELISA

Antigène: Ghrelin (GHRL)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 8-2000 pg/mL

Seuil minimal de détection: 8 pg/mL

Application: ELISA

Fonction: Human Ghrelin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Ghrelin (GHRL)

Sensibilité: 8 pg/mL.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Ghrelin (GHRL)

Autre désignation: GHRL (GHRL Produits)

Synonymes: 2210006E23Rik Kit ELISA, Ghr Kit ELISA, MTLRP Kit ELISA, MTLRPAP Kit ELISA, m46 Kit ELISA, Gh1-1 Kit ELISA, GRHL Kit ELISA, MTLRP-AP Kit ELISA, GHR Kit ELISA, ghrelin Kit ELISA, ghrelin and obestatin prepropeptide Kit ELISA, ghrelin/obestatin prepropeptide Kit ELISA, Ghrl Kit ELISA, GHRL Kit ELISA, ghrl Kit ELISA

Sujet: Gene Names: GHRL MTLRP UNQ524/PRO1066
Protein names: Appetite-regulating hormone (Growth hormone secretagogue) (Growth hormone-releasing peptide) (Motilin-related peptide) (Protein M46) [Cleaved into: Ghrelin-27, Ghrelin-28 (Ghrelin), Obestatin]

ID gène: 51738

UniProt: Q9UBU3

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour