IGFBP7 Kit ELISA

N° du produit ABIN2703131

Détail du produit IGFBP7 Kit ELISA

Antigène: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.065-50 ng/mL

Seuil minimal de détection: 0.065 ng/mL

Application: ELISA

Fonction: Human IGFBP-rp1 (IGFBP-7) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human IGFBP-rp1. Other species not yet determined.

Sensibilité: 0.065 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples100 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Autre désignation: IGFBP-Rp1/IGFBP-7 (IGFBP7 Produits)

Synonymes: zgc:85888 Kit ELISA, AGM Kit ELISA, FSTL2 Kit ELISA, IBP-7 Kit ELISA, IGFBP-7 Kit ELISA, IGFBP-7v Kit ELISA, IGFBPRP1 Kit ELISA, MAC25 Kit ELISA, PSF Kit ELISA, RAMSVPS Kit ELISA, TAF Kit ELISA, Fstl2 Kit ELISA, Mac25 Kit ELISA, insulin like growth factor binding protein 7 Kit ELISA, insulin-like growth factor binding protein 7 Kit ELISA, IGFBP7 Kit ELISA, igfbp7 Kit ELISA, Igfbp7 Kit ELISA

Sujet: Gene Names: IGFBP7 MAC25 PSF
Protein names: Insulin-like growth factor-binding protein 7 (IBP-7) (IGF-binding protein 7) (IGFBP-7) (IGFBP-rP1) (MAC25 protein) (PGI2-stimulating factor) (Prostacyclin-stimulating factor) (Tumor-derived adhesion factor) (TAF)

ID gène: 3490

UniProt: Q16270

Pathways: Growth Factor Binding