IL1F9 Kit ELISA

N° du produit ABIN2703199

Ce kit ELISA Humain IL1F9 est un kit ELISA Colorimetric conçu pour quantifier Humain IL1F9.

Aperçu rapide pour IL1F9 Kit ELISA (ABIN2703199)

Antigène: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 13-3000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Détail du produit IL1F9 Kit ELISA

Seuil minimal de détection: 13 pg/mL

Fonction: Human IL-36 gamma (IL-1 F9) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F7 (IL-37), IL-1F8 (IL-36 beta), IL-1F10 (IL-38), IL-1F11 (IL-33)

Sensibilité: 13 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Autre désignation: IL-1F9

Sujet: Gene Names: IL36G IL1E IL1F9 IL1H1 IL1RP2 UNQ2456/PRO5737
Protein names: Interleukin-36 gamma (IL-1-related protein 2) (IL-1RP2) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 9) (IL-1F9) (Interleukin-1 homolog 1) (IL-1H1)

ID gène: 56300

UniProt: Q9NZH8