Periostin Kit ELISA

N° du produit ABIN2703411

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris Periostin.

Aperçu rapide pour Periostin Kit ELISA (ABIN2703411)

Antigène: Periostin (POSTN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.05-16 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit Periostin Kit ELISA

Seuil minimal de détection: 0.05 ng/mL

Fonction: Mouse Periostin ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17E, IL-17F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.

Sensibilité: 0.05 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples50 - 500 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

Collect plasma using heparin and/or EDTA as the anticoagulant. Citrate is not recommended.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Periostin (POSTN)

Autre désignation: Periostin

Sujet: Gene Names: Postn Osf2
Protein names: Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)

ID gène: 50706

UniProt: Q62009