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PGLYRP1 Kit ELISA
PGLYRP1
Reactivité: Humain
Colorimetric
Sandwich ELISA
6-1.500 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703415
Détail du produit PGLYRP1 Kit ELISA
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Antigène
Voir toutes PGLYRP1 Kits ELISA
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Reactivité
Toutes les réactivités sur PGLYRP1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
6-1.500 pg/mL
Seuil minimal de détection
6 pg/mL
Application
ELISA
Fonction
Human PGRPs ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair detects human PGRPs, less than 0.05 % cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.
Sensibilité
6 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples100 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes PGLYRP1 Kits ELISA
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Autre désignation
PGRPs (PGLYRP1 Produits )
Synonymes
PGLYRP1 Kit ELISA, PGLYRP Kit ELISA, pgrp Kit ELISA, tag7 Kit ELISA, pgrps Kit ELISA, pglyrp Kit ELISA, pgrp-s Kit ELISA, tnfsf3l Kit ELISA, MGC108330 Kit ELISA, MGC108469 Kit ELISA, PGRP-SC1a Kit ELISA, si:dkey-113g17.4 Kit ELISA, si:ch211-202c21.3 Kit ELISA, PGRP Kit ELISA, PGRP-S Kit ELISA, PGRPS Kit ELISA, TAG7 Kit ELISA, TNFSF3L Kit ELISA, Pglyrp Kit ELISA, Tag7 Kit ELISA, Tasg7 Kit ELISA, Tnfsf3l Kit ELISA, Pgrp Kit ELISA, peptidoglycan recognition protein 1 Kit ELISA, peptidoglycan recognition protein 1 L homeolog Kit ELISA, peptidoglycan recognition protein 5 Kit ELISA, PGLYRP1 Kit ELISA, pglyrp1.L Kit ELISA, pglyrp1 Kit ELISA, pglyrp5 Kit ELISA, Pglyrp1 Kit ELISA
Sujet
Gene Names: PGLYRP1 PGLYRP PGRP TNFSF3L SBBI68 UNQ639/PRO1269 Protein names: Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S)
ID gène
8993
UniProt
O75594
Pathways
Activation of Innate immune Response
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