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SHBG Kit ELISA

SHBG Reactivité: Humain Colorimetric Sandwich ELISA 1.2-300 pM/L Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703457
  • Antigène Voir toutes SHBG Kits ELISA
    SHBG (Sex Hormone Binding Globulin (SHBG))
    Reactivité
    • 6
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.2-300 pM/L
    Seuil minimal de détection
    1.2 pM/L
    Application
    ELISA
    Fonction
    Human SHBG ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects human SHBG. Other species not determined.
    Sensibilité
    1.2 pmol/L
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples1,500 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes SHBG Kits ELISA
    SHBG (Sex Hormone Binding Globulin (SHBG))
    Autre désignation
    SHBG (SHBG Produits)
    Synonymes
    ABP Kit ELISA, SBP Kit ELISA, TEBG Kit ELISA, Abpa Kit ELISA, shbgl Kit ELISA, wu:fb66h07 Kit ELISA, wu:fd55b08 Kit ELISA, zgc:123063 Kit ELISA, shbg Kit ELISA, sex hormone binding globulin Kit ELISA, sex hormone-binding globulin Kit ELISA, sex hormone binding globulin L homeolog Kit ELISA, SHBG Kit ELISA, Shbg Kit ELISA, shbg Kit ELISA, shbg.L Kit ELISA
    Sujet
    Gene Names: SHBG
    Protein names: Sex hormone-binding globulin (SHBG) (Sex steroid-binding protein) (SBP) (Testis-specific androgen-binding protein) (ABP) (Testosterone-estradiol-binding globulin) (TeBG) (Testosterone-estrogen-binding globulin)
    ID gène
    6462
    UniProt
    P04278
    Pathways
    Hormone Transport
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