SPARC Kit ELISA

N° du produit ABIN2708375

Le kit ELISA SPARC (ABIN2708375) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain SPARC.

Aperçu rapide pour SPARC Kit ELISA (ABIN2708375)

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit SPARC Kit ELISA

Seuil minimal de détection: 625 pg/mL

Fonction: Sandwich ELISA for quantitative detection of Human SPARC concentrations.

Analytical Method: Quantitative

Sensibilité: 235 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Date de péremption: 6-12 months

Détail du antigène

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC

Sujet: Alternative Names: ON, SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine

ID gène: 6678

UniProt: P09486

Pathways: Autophagy