ADIPOQ Kit ELISA

N° du produit ABIN2747931

Détail du produit ADIPOQ Kit ELISA

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.8-200 ng/mL

Seuil minimal de détection: 0.8 ng/mL

Application: ELISA

Fonction: Rat Adiponectin (ACRP30) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rat Adiponectin (ADIPOQ/ACRP30)

Sensibilité: 0.8 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour ADIPOQ Kit ELISA (ABIN2747931)

Thakare, Surana: "β-Asarone modulate adipokines and attenuates high fat diet-induced metabolic abnormalities in Wistar rats." dans: Pharmacological research, Vol. 103, pp. 227-35, (2016) (PubMed).

Shihabudeen, Roy, James, Thirumurugan: "Chenodeoxycholic acid, an endogenous FXR ligand alters adipokines and reverses insulin resistance." dans: Molecular and cellular endocrinology, Vol. 414, pp. 19-28, (2015) (PubMed).

Détail du antigène

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Autre désignation: Adiponectin (ADIPOQ Produits)

Synonymes: ADP Kit ELISA, DCAF9 Kit ELISA, 6720489L24Rik Kit ELISA, 9330209L04Rik Kit ELISA, Adp Kit ELISA, Mamdc1 Kit ELISA, RNSVSP4U Kit ELISA, SVSIV1 Kit ELISA, Svp4 Kit ELISA, ACDC Kit ELISA, ACRP30 Kit ELISA, ADIPQTL1 Kit ELISA, ADPN Kit ELISA, APM-1 Kit ELISA, APM1 Kit ELISA, GBP28 Kit ELISA, apM-1 Kit ELISA, 30kDa Kit ELISA, APN Kit ELISA, Acdc Kit ELISA, Acrp30 Kit ELISA, Ad Kit ELISA, adipo Kit ELISA, apM1 Kit ELISA, ADN Kit ELISA, acrpl Kit ELISA, adipo-b Kit ELISA, adipoql Kit ELISA, zgc:153584 Kit ELISA, adipoq Kit ELISA, WD and tetratricopeptide repeats 1 Kit ELISA, MAM domain containing glycosylphosphatidylinositol anchor 2 Kit ELISA, seminal vesicle secretory protein 4 Kit ELISA, adiponectin, C1Q and collagen domain containing Kit ELISA, adiponectin, C1Q and collagen domain containing, b Kit ELISA, adiponectin, C1Q and collagen domain containing L homeolog Kit ELISA, WDTC1 Kit ELISA, Mdga2 Kit ELISA, Svs4 Kit ELISA, ADIPOQ Kit ELISA, Adipoq Kit ELISA, adipoqb Kit ELISA, adipoq.L Kit ELISA

Sujet: Gene Names: Adipoq Acdc rCG_36676
Protein names: 30 kDa adipocyte complement-related protein (Adiponectin, C1Q and collagen domain containing) (Protein Adipoq)

ID gène: 246253

UniProt: Q8K3R4