CCL27 Kit ELISA

N° du produit ABIN2748031

Détail du produit CCL27 Kit ELISA

Antigène: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rhesus Macaque Cutaneous T-cell Attracting Chemokine (CCL27) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rhesus Macaque Cutaneous T-cell Attracting Chemokine (CCL27)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Autre désignation: CTACK (CCL27 Produits)

Synonymes: ALP Kit ELISA, ILC Kit ELISA, CTACK Kit ELISA, ESkine Kit ELISA, CCL27 Kit ELISA, CTAK Kit ELISA, ESKINE Kit ELISA, PESKY Kit ELISA, SCYA27 Kit ELISA, AW558992 Kit ELISA, Ccl27 Kit ELISA, Scya27 Kit ELISA, Scya27a Kit ELISA, C-C motif chemokine ligand 27 Kit ELISA, uncharacterized LOC508933 Kit ELISA, chemokine (C-C motif) ligand 27A Kit ELISA, Ccl27 Kit ELISA, CCL27 Kit ELISA, LOC508933 Kit ELISA, Ccl27a Kit ELISA

Sujet: Cutaneous T-cell Attracting Chemokine (CCL27)

ID gène: 574220

UniProt: Q8HYP2