LGALS1/Galectin 1 Kit ELISA

N° du produit ABIN2748113

Kit ELISA LGALS1/Galectin 1 Humain et Rat, Colorimetric test pour la quantification de Humain et Rat LGALS1/Galectin 1.

Aperçu rapide pour LGALS1/Galectin 1 Kit ELISA (ABIN2748113)

Antigène: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivité: Humain, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15 ng/mL - 25 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit LGALS1/Galectin 1 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Fonction: Rat Galectin-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB (TNFRSF9/CD137), Activin A, Adiponectin (ACRP30), CD80 (B7-1), CD48 (SLAMF2), CTACK (CCL27), Decorin, Eotaxin-1 (CCL11), EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-3, Gas 1, GFR alpha-1 (GDNF R), gp130, HGF, IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A (CD321/F11R), MIP-1 alpha (CCL3), Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES (CCL5), SCF, TIM1 (KIM-1), TIMP-2, TREM-1, TWEAK R (TNFRSF12).

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Autre désignation: Galectin-1

Sujet:

Gene Symbols: LGALS1

Protein Name (Synonyms): Galectin-1 (Gal-1) (14 kDa lectin) (Beta-galactoside-binding lectin L-14-I) (Galaptin) (Lactose-binding lectin 1) (Lectin galactoside-binding soluble 1) (RL 14.5) (S-Lac lectin 1)

ID gène: 56646

UniProt: P11762

Pathways: Carbohydrate Homeostasis