GAS1 Kit ELISA

N° du produit ABIN2748128

Détail du produit GAS1 Kit ELISA

Antigène: GAS1 (Growth Arrest-Specific 1 (GAS1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rat Growth Arrest-Specific 1 (GAS1) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rat Growth Arrest-Specific 1 (GAS1)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GAS1 (Growth Arrest-Specific 1 (GAS1))

Autre désignation: Gas 1 (GAS1 Produits)

Synonymes: AW554192 Kit ELISA, Gas-1 Kit ELISA, MGC147541 Kit ELISA, id:ibd5013 Kit ELISA, wu:fa11f02 Kit ELISA, wu:faa26f09 Kit ELISA, im:7428747 Kit ELISA, wu:fc54h12 Kit ELISA, zgc:153938 Kit ELISA, growth arrest specific 1 Kit ELISA, growth arrest-specific 1 Kit ELISA, growth arrest-specific 1a Kit ELISA, growth arrest-specific 1b Kit ELISA, GAS1 Kit ELISA, Gas1 Kit ELISA, gas1 Kit ELISA, gas1a Kit ELISA, gas1b Kit ELISA

Sujet: Growth Arrest-Specific 1 (GAS1)

ID gène: 683470

UniProt: M0RBH9