CCL4 Kit ELISA

N° du produit ABIN2748349

Détail du produit CCL4 Kit ELISA

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rhesus Macaque MIP-1 beta (CCL4) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rhesus Macaque MIP-1 beta (CCL4)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Autre désignation: MIP-1 beta (CCL4 Produits)

Synonymes: ACT2 Kit ELISA, AT744.1 Kit ELISA, G-26 Kit ELISA, HC21 Kit ELISA, LAG-1 Kit ELISA, LAG1 Kit ELISA, MIP-1-beta Kit ELISA, MIP1B Kit ELISA, MIP1B1 Kit ELISA, SCYA2 Kit ELISA, SCYA4 Kit ELISA, CCL4 Kit ELISA, K203 Kit ELISA, Mip1-b Kit ELISA, Scya4 Kit ELISA, MIP-1beta Kit ELISA, MIP-1BETA Kit ELISA, MIP1b Kit ELISA, SCY4A Kit ELISA, Act-2 Kit ELISA, MIP-1B Kit ELISA, Mip1b Kit ELISA, ACT-2 Kit ELISA, AT744.2 Kit ELISA, CCL4L Kit ELISA, SCYA4L Kit ELISA, SCYA4L1 Kit ELISA, SCYA4L2 Kit ELISA, C-C motif chemokine ligand 4 Kit ELISA, chemokine (C-C motif) ligand 4 Kit ELISA, chemokine (C-C motif) ligand 4-like 1 Kit ELISA, C-C motif chemokine ligand 4 like 1 Kit ELISA, CCL4 Kit ELISA, Ccl4 Kit ELISA, CCL4L1 Kit ELISA

Sujet: MIP-1 beta (CCL4)

ID gène: 574217

UniProt: Q8HYQ2