TREM1 Kit ELISA

N° du produit ABIN2859211

Kit ELISA Souris TREM1, test Colorimetric pour la quantification de Souris TREM1.

Aperçu rapide pour TREM1 Kit ELISA (ABIN2859211)

Antigène: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Épitope: AA 21-202

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Cell Lysate, Tissue Homogenate

Détail du produit TREM1 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TREM-1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A21-S202

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A21-S202

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse TREM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TREM-1 has been precoated onto 96-well plates. Standards(NSO, A21-S202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TREM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TREM-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TREM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA), cell lysates or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TREM-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 252, Standard deviation: 14.93, CV(%): 5.9
• Sample 2: n=16, Mean(pg/ml): 559, Standard deviation: 39.13, CV(%): 7
• Sample 3: n=16, Mean(pg/ml): 924, Standard deviation: 42.5, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 314, Standard deviation: 24.5, CV(%): 7.8
• Sample 2: n=24, Mean(pg/ml): 675, Standard deviation: 48.6, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 1104, Standard deviation: 60.72, CV(%): 5.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Autre désignation: TREM1

Sujet:

Protein Function: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity). .

Background: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF), MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

Synonyms: Triggering receptor expressed on myeloid cells 1,TREM-1,CD354,Trem1,

Full Gene Name: Triggering receptor expressed on myeloid cells 1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 58217

UniProt: Q9JKE2