CD93 Kit ELISA

N° du produit ABIN2859214

Kit ELISA Humain CD93, test Colorimetric pour la quantification de Humain CD93.

Aperçu rapide pour CD93 Kit ELISA (ABIN2859214)

Antigène: CD93

Épitope: AA 24-580

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78-5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Détail du produit CD93 Kit ELISA

Seuil minimal de détection: 78 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD93/C1qR

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A24-K580

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A24-K580

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 C-type lectin domain.

Tissue Specificity: Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.

Plaque: Pre-coated

Protocole: human CD93 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD93 has been precoated onto 96-well plates. Standards(NSO, A24-K580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD93 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD93 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CD93 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD93 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 634, Standard deviation: 33.6, CV(%): 5.3
• Sample 2: n=16, Mean(pg/ml): 1493, Standard deviation: 92.6, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 2436, Standard deviation: 107.2, CV(%): 4.4,
• Sample 1: n=24, Mean(pg/ml): 572, Standard deviation: 38.9, CV(%): 6.8
• Sample 2: n=24, Mean(pg/ml): 1620, Standard deviation: 121.5, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 2864, Standard deviation: 160.4, CV(%): 5.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CD93

Autre désignation: CD93

Sujet:

Protein Function: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.

Background: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

Synonyms: Complement component C1q receptor,C1q/MBL/SPA receptor,C1qR,C1qR(p),C1qRp,CDw93,Complement component 1 q subcomponent receptor 1,Matrix-remodeling-associated protein 4,CD93,CD93,C1QR1, MXRA4,

Full Gene Name: Complement component C1q receptor

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 22918

UniProt: Q9NPY3