IL-20 Kit ELISA

N° du produit ABIN2859222

Le kit ELISA IL-20 (ABIN2859222) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris IL-20. Ce kit ELISA a été cité dans 3 publications.

Aperçu rapide pour IL-20 Kit ELISA (ABIN2859222)

Antigène: IL-20 (IL20) (Interleukin 20 (IL20))

Épitope: AA 25-176

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit IL-20 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Mouse IL-20 ELISA Kit to quantitate Mouse Il20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: L25-E176

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6.9%, 5%, 6.7%
Inter-Assay Precision (CV%): 7.8%, 6.2%, 7.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour IL-20 Kit ELISA (ABIN2859222)

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." dans: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Zhou, Jin, Jia, Zhang, Li, Liu, Liu: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." dans: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." dans: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, (2012) (PubMed).

Détail du antigène

Antigène: IL-20 (IL20) (Interleukin 20 (IL20))

Autre désignation: IL20

Sujet:

Background: IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Gene Full Name: interleukin 20

ID gène: 58181

UniProt: Q9JKV9

Pathways: Protein targeting to Nucleus