SDC4 Kit ELISA

N° du produit ABIN2859227

Détail du produit SDC4 Kit ELISA

Antigène: SDC4 (Syndecan 4 (SDC4))

Épitope: AA 19-145

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Syndecan-4

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: E19-E145

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: E19-E145

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed in epithelial and fibroblastic cells. .

Plaque: Pre-coated

Protocole: human Syndecan-4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-4 has been precoated onto 96-well plates. Standards(NSO, E19-E145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-4 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Syndecan-4 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 630, Standard deviation: 26.5, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1447, Standard deviation: 83.9, CV(%): 5.8
• Sample 3: n=16, Mean(pg/ml): 2135, Standard deviation: 100.3, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 718, Standard deviation: 35.2, CV(%): 4.9
• Sample 2: n=24, Mean(pg/ml): 1604, Standard deviation: 104.3, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 2369, Standard deviation: 120.8, CV(%): 5.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: SDC4 (Syndecan 4 (SDC4))

Autre désignation: SDC4 (SDC4 Produits)

Synonymes: SYND4 Kit ELISA, AA959608 Kit ELISA, AW108331 Kit ELISA, Synd4 Kit ELISA, ryudocan Kit ELISA, syndecan-4 Kit ELISA, RATRYUDOCA Kit ELISA, RYUDOCA Kit ELISA, SDC4 Kit ELISA, sdc4l Kit ELISA, wu:fc47g09 Kit ELISA, Syndecan-4 Kit ELISA, amphiglycan Kit ELISA, syn-4 Kit ELISA, syn4 Kit ELISA, syndecan 4 Kit ELISA, syndecan 4 S homeolog Kit ELISA, syndecan 4 L homeolog Kit ELISA, SDC4 Kit ELISA, Sdc4 Kit ELISA, sdc4 Kit ELISA, sdc4.S Kit ELISA, sdc4.L Kit ELISA

Sujet:

Protein Function: Cell surface proteoglycan that bears heparan sulfate.

Background: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

Synonyms: Syndecan-4,SYND4,Amphiglycan,Ryudocan core protein,SDC4,

Full Gene Name: Syndecan-4

Cellular Localisation: Isoform 1: Membrane, Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form..

ID gène: 6385

UniProt: P31431

Pathways: Glycosaminoglycan Metabolic Process