CEACAM1 Kit ELISA

N° du produit ABIN2859230

Détail du produit CEACAM1 Kit ELISA

Antigène: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Épitope: AA 35-428

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20.000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CEACAM1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q35-G428

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q35-G428

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the immunoglobulin superfamily. CEA family.

Plaque: Pre-coated

Protocole: human CEACAM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEACAM1 has been precoated onto 96-well plates. Standards(NSO, Q35-G428) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEACAM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CEACAM1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEACAM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CEACAM1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 5.14, Standard deviation: 0.185, CV(%): 3.6
• Sample 2: n=16, Mean(ng/ml): 7.35, Standard deviation: 0.397, CV(%): 5.4
• Sample 3: n=16, Mean(ng/ml): 12.1, Standard deviation: 0.593, CV(%): 4.9,
• Sample 1: n=24, Mean(ng/ml): 5.36, Standard deviation: 0.295, CV(%): 5.5
• Sample 2: n=24, Mean(ng/ml): 8.04, Standard deviation: 0.571, CV(%): 7.1
• Sample 3: n=24, Mean(ng/ml): 13.5, Standard deviation: 0.837, CV(%): 6.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Autre désignation: CEACAM1 (CEACAM1 Produits)

Synonymes: BGP Kit ELISA, BGP1 Kit ELISA, BGPI Kit ELISA, CD66e Kit ELISA, CEA Kit ELISA, zeh0068 Kit ELISA, hm:zeh0068 Kit ELISA, CEACAM8 Kit ELISA, CEACAM1 Kit ELISA, MGC142545 Kit ELISA, BGPR Kit ELISA, Bgp Kit ELISA, CD66a Kit ELISA, Ccam1 Kit ELISA, Bgp1 Kit ELISA, C-CAM Kit ELISA, Cc1 Kit ELISA, Cea-1 Kit ELISA, Cea-7 Kit ELISA, Cea1 Kit ELISA, Cea7 Kit ELISA, Hv-2 Kit ELISA, Hv2 Kit ELISA, MHVR Kit ELISA, MHVR1 Kit ELISA, Mhv-1 Kit ELISA, bb-1 Kit ELISA, mCEA1 Kit ELISA, mmCGM1 Kit ELISA, mmCGM1a Kit ELISA, mmCGM2 Kit ELISA, carcinoembryonic antigen related cell adhesion molecule 1 Kit ELISA, carcinoembryonic antigen related cell adhesion molecule 5 Kit ELISA, carcinoembryonic antigen-related cell adhesion molecule 1 Kit ELISA, carcinoembryonic antigen-related cell adhesion molecule 6 Kit ELISA, CEACAM1 Kit ELISA, CEACAM5 Kit ELISA, ceacam1 Kit ELISA, Ceacam1 Kit ELISA, LOC101103343 Kit ELISA

Sujet:

Background: CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.

Synonyms: Carcinoembryonic antigen-related cell adhesion molecule 1,Biliary glycoprotein 1,BGP-1,CD66a,CEACAM1,BGP, BGP1,

Full Gene Name: Carcinoembryonic antigen-related cell adhesion molecule 1

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

ID gène: 634

UniProt: P13688