CXCL3 Kit ELISA

N° du produit ABIN2859233

Détail du produit CXCL3 Kit ELISA

Antigène: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Épitope: AA 32-100

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL3

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: E.coli, S32-S100

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: S32-S100

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL3 has been precoated onto 96-well plates. Standards(E.coli, S32-S100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL3 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL3 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 178, Standard deviation: 7.3, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 304, Standard deviation: 11.86, CV(%): 3.9
• Sample 3: n=16, Mean(pg/ml): 569, Standard deviation: 30.2, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 38.2, Standard deviation: 2.56, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 128, Standard deviation: 8.70, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 642, Standard deviation: 49.43, CV(%): 7.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CXCL3 Kit ELISA (ABIN2859233)

Pena-Philippides, Caballero-Garrido, Lordkipanidze, Roitbak: "In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response." dans: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 287, (2017) (PubMed).

Détail du antigène

Antigène: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Autre désignation: CXCL3 (CXCL3 Produits)

Sujet:

Protein Function: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .

Background: Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Synonyms: C-X-C motif chemokine 3,Dendritic cell inflammatory protein 1 ,Cxcl3 ,Dcip1 , Gm1960 ,

Full Gene Name: C-X-C motif chemokine 3

Cellular Localisation: Secreted.

ID gène: 330122

UniProt: Q6W5C0

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy