SERPINC1 Kit ELISA

N° du produit ABIN2859247

Kit ELISA Humain SERPINC1, test Colorimetric pour la quantification de Humain SERPINC1.

Aperçu rapide pour SERPINC1 Kit ELISA (ABIN2859247)

Antigène: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Épitope: AA 33-464

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20.000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Détail du produit SERPINC1 Kit ELISA

Seuil minimal de détection: 312 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Serpin C1/Antithrombin-III

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: NSO, H33-K464

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: H33-K464

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Found in plasma.

Plaque: Pre-coated

Protocole: human Serpin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin C1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.05, Standard deviation: 0.064, CV(%): 6.1
• Sample 2: n=16, Mean(ng/ml): 3.42, Standard deviation: 0.194, CV(%): 5.7
• Sample 3: n=16, Mean(ng/ml): 8.67, Standard deviation: 0.546, CV(%): 6.3,
• Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.088, CV(%): 6.7
• Sample 2: n=24, Mean(ng/ml): 3.74, Standard deviation: 0.239, CV(%): 6.4
• Sample 3: n=24, Mean(ng/ml): 8.23, Standard deviation: 0.543, CV(%): 6.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Autre désignation: SERPINC1

Sujet:

Protein Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. .

Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22 % of the intron sequence) than in the genome as a whole (about 5 %).

Synonyms: Antithrombin-III,ATIII,Serpin C1,SERPINC1,AT3,PRO0309,

Full Gene Name: Antithrombin-III

Cellular Localisation: Secreted, extracellular space.

ID gène: 462

UniProt: P01008