Relaxin 3 Kit ELISA

N° du produit ABIN2859258

Ce kit ELISA Humain Relaxin 3 est un kit ELISA Colorimetric conçu pour quantifier Humain Relaxin 3.

Aperçu rapide pour Relaxin 3 Kit ELISA (ABIN2859258)

Antigène: Relaxin 3 (RLN3)

Épitope: AA 27-53, AA 119-142

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit Relaxin 3 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Relaxin 3

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: E.coli, D119-C142(A)&A27-R53(B)

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: D119-C142(A)&A27-R53(B)

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human Relaxin 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 3 has been precoated onto 96-well plates. Standards(E. coli, D119-C142 (A) & A27-R53(B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 3 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Relaxin 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Relaxin 3 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 6.16, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 259, Standard deviation: 13.20, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 973, Standard deviation: 50.59, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 7.75, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 276, Standard deviation: 16.28, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 1093, Standard deviation: 60.11, CV(%): 5.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Relaxin 3 (RLN3)

Autre désignation: RLN3

Sujet:

Protein Function: May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).

Background: Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory, and likely plays a role in the generation/regulation of hippocampal theta rhythm.

Synonyms: Relaxin-3,Insulin-like peptide INSL7,Insulin-like peptide 7,Prorelaxin H3,Relaxin-3 B chain,Relaxin-3 A chain,RLN3,INSL7, RXN3, ZINS4,UNQ6188/PRO20213,

Full Gene Name: Relaxin-3

Cellular Localisation: Secreted.

ID gène: 117579

UniProt: Q8WXF3

Pathways: Hormone Activity, cAMP Metabolic Process