IL17D Kit ELISA

N° du produit ABIN2859299

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris IL17D.

Aperçu rapide pour IL17D Kit ELISA (ABIN2859299)

Antigène: IL17D (Interleukin 17D (IL17D))

Épitope: AA 25-205

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit IL17D Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17D

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A25-R205

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A25-R205

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-17D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been precoated onto 96-well plates. Standards(E.coli, A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17D amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-17D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17D standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 715, Standard deviation: 30.03, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1410, Standard deviation: 64.86, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 2703, Standard deviation: 137.9, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 694, Standard deviation: 38.17, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 1582, Standard deviation: 99.67, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 2691, Standard deviation: 180.3, CV(%): 6.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: IL17D (Interleukin 17D (IL17D))

Autre désignation: IL17D

Sujet:

Background: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.

Synonyms: IL-17D ,Il17d ,

Full Gene Name: interleukin 17D

ID gène: 239114

UniProt: Q8K4C4

Pathways: Cellular Response to Molecule of Bacterial Origin