IL-25 Kit ELISA

N° du produit ABIN2859300

Détail du produit IL-25 Kit ELISA

Antigène: IL-25 (IL25) (Interleukin 25 (IL25))

Épitope: AA 33-177

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17E/IL-25

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: Y33-G177

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: Y33-G177

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the IL-17 family.

Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

Plaque: Pre-coated

Protocole: human IL-17E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been precoated onto 96-well plates. Standards(E.coli, Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17E amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-17E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-17E standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 621, Standard deviation: 24.2, CV(%): 3.9
• Sample 2: n=16, Mean(pg/ml): 1969, Standard deviation: 90.6, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 2795, Standard deviation: 150.9, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 730, Standard deviation: 30.7, CV(%): 4.2
• Sample 2: n=24, Mean(pg/ml): 2103, Standard deviation: 119.9, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 3065, Standard deviation: 208.4, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-25 Kit ELISA (ABIN2859300)

Meng, Shen, Shen, Zhou, Shen, Das: "Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro." dans: Lipids in health and disease, Vol. 12, pp. 160, (2014) (PubMed).

Détail du antigène

Antigène: IL-25 (IL25) (Interleukin 25 (IL25))

Autre désignation: IL25 (IL25 Produits)

Synonymes: IL17E Kit ELISA, IL-17e Kit ELISA, Il17e Kit ELISA, IL25 Kit ELISA, RGD1561632 Kit ELISA, interleukin 25 Kit ELISA, IL25 Kit ELISA, Il25 Kit ELISA

Sujet:

Protein Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Background: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-i°B activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

Synonyms: Interleukin-25,IL-25,Interleukin-17E,IL-17E,IL25,IL17E,UNQ3120/PRO10272,

Full Gene Name: Interleukin-25

Cellular Localisation: Secreted.

ID gène: 64806

UniProt: Q9H293