PDGF-BB Homodimer Kit ELISA

N° du produit ABIN2859308

Détail du produit

Antigène: PDGF-BB Homodimer

Épitope: AA 82-190

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: For quantitative detection of human PDGF-BB in cell culture supernates, serum and plasma(heparin, EDTA).

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant human PDGF-BB

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Ingrédients:

• 96-well plate precoated with anti- human PDGF-BB antibod - 1
• Lyophilized recombinant human PDGF-BB standar - 10ng/tubex2
• Biotinylated anti- human PDGF-BB antibod - 130μl(dilution 1:100)
• Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
• Sample diluent buffe - 30 ml
• Antibody diluent buffe - 12ml
• ABC diluent buffe - 12ml
• TMB color developing agen - 10ml
• TMB stop solutio - 10ml

Immunogène: Expression system for standard: E.coli
Immunogen sequence: S82-T190

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: human PDGF-BB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-BB has been precoated onto 96-well plates. Standards(E.coli, S82-T190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-BB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PDGF-BB amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PDGF-BB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PDGF-BB standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 18.5, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 818, Standard deviation: 38.5, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 1163, Standard deviation: 71, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 25.43, CV(%): 7.8
• Sample 2: n=24, Mean(pg/ml): 794, Standard deviation: 60.4, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 1234, Standard deviation: 105, CV(%): 8.5

Restrictions: For Research Use only

Stockage

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Référence

Dai, Cui, Zhang, Cheng, Liu, Yang, Fan, Wang, Lin, Zhang, Li, Mao, Wang, Su, Zhang, Peng, Yang, Hu, Yang, Huang, Xiang, Yu, Zhou, Wei, Deng: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." dans: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).

Liang, Wang, Zhang, Lu, Wang: "Expression and functional analysis of platelet-derived growth factor in uterine leiomyomata." dans: Cancer biology & therapy, Vol. 5, Issue 1, pp. 28-33, (2006) (PubMed).

Détail du antigène

Antigène: PDGF-BB Homodimer

Autre désignation: PDGF-BB

Synonymes: PDGF-2 Kit ELISA, PDGF2 Kit ELISA, SIS Kit ELISA, SSV Kit ELISA, c-sis Kit ELISA, PDGF-B Kit ELISA, Sis Kit ELISA, platelet derived growth factor subunit B Kit ELISA, platelet derived growth factor, B polypeptide Kit ELISA, PDGFB Kit ELISA, Pdgfb Kit ELISA

Sujet: Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer(PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide(PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

ID gène: 5155

UniProt: P01127