SPARCL1 Kit ELISA

N° du produit ABIN2859327

Ce kit ELISA Humain SPARCL1 est un kit ELISA Colorimetric conçu pour quantifier Humain SPARCL1.

Aperçu rapide pour SPARCL1 Kit ELISA (ABIN2859327)

Antigène: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Épitope: AA 17-664

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.9-3000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit SPARCL1 Kit ELISA

Seuil minimal de détection: 46.9 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SPARCL1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: I17-F664

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: I17-F664

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the SPARC family.

Tissue Specificity: Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.

Plaque: Pre-coated

Protocole: human SPARCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been precoated onto 96-well plates. Standards(NSO, I17-F664) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SPARCL1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL human SPARCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human SPARCL1 standard solution and each sample is measured in duplicate. 2. Seal the plate with the cover and incubate at 37 °C for 90 min.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 269, Standard deviation: 11.03, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 1195, Standard deviation: 57.36, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 2030, Standard deviation: 105.6, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 321, Standard deviation: 18.3, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 1254, Standard deviation: 79, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 2208, Standard deviation: 167.8, CV(%): 7.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Autre désignation: SPARCL1

Sujet:

Background: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Synonyms: SPARC-like protein 1,High endothelial venule protein,Hevin,MAST 9,SPARCL1,

Full Gene Name: SPARC-like protein 1

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 8404

UniProt: Q14515