DKK3 Kit ELISA

N° du produit ABIN2859331

Kit ELISA Humain DKK3, test Colorimetric pour la quantification de Humain DKK3.

Aperçu rapide pour DKK3 Kit ELISA (ABIN2859331)

Antigène: DKK3

Épitope: AA 22-350

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit DKK3 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DKK-3

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A22-I350

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A22-I350

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Highest expression in heart, brain, and spinal cord. .

Plaque: Pre-coated

Protocole: human DKK-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK-3 has been precoated onto 96-well plates. Standards(NSO, A22-I350) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK-3 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human DKK-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human DKK-3 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1228, Standard deviation: 79.82, CV(%): 6.5
• Sample 2: n=16, Mean(pg/ml): 3386, Standard deviation: 169.3, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 5833, Standard deviation: 332.5, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 1439, Standard deviation: 110.8, CV(%): 7.7
• Sample 2: n=24, Mean(pg/ml): 3627, Standard deviation: 196, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 6294, Standard deviation: 428, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: DKK3

Sujet:

Protein Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). .

Background: Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Synonyms: Dickkopf-related protein 3,Dickkopf-3,Dkk-3,hDkk-3,DKK3,REIC,UNQ258/PRO295,

Full Gene Name: Dickkopf-related protein 3

Cellular Localisation: Secreted.

ID gène: 27122

UniProt: Q9UBP4