ADIPOQ Kit ELISA

N° du produit ABIN2859334

Détail du produit ADIPOQ Kit ELISA

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Épitope: AA 19-244

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Adiponectin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: I19-N244

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: I19-N244

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: rat Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(E.coli, I19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Adiponectin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Adiponectin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.6, Standard deviation: 0.027, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.112, CV(%): 3.2
• Sample 3: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.261, CV(%): 3.9,
• Sample 1: n=24, Mean(ng/ml): 1.2, Standard deviation: 0.114, CV(%): 9.5
• Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.277, CV(%): 7.1
• Sample 3: n=24, Mean(ng/ml): 5.7, Standard deviation: 0.49, CV(%): 8.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour ADIPOQ Kit ELISA (ABIN2859334)

El Tabaa, Sokkar, Ramadan, Abd El Salam, Zaid: "Neuroprotective role of Ginkgo biloba against cognitive deficits associated with Bisphenol A exposure: An animal model study." dans: Neurochemistry international, Vol. 108, pp. 199-212, (2017) (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." dans: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." dans: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." dans: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." dans: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." dans: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).

Détail du antigène

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Autre désignation: ADIPOQ (ADIPOQ Produits)

Synonymes: ADP Kit ELISA, DCAF9 Kit ELISA, 6720489L24Rik Kit ELISA, 9330209L04Rik Kit ELISA, Adp Kit ELISA, Mamdc1 Kit ELISA, RNSVSP4U Kit ELISA, SVSIV1 Kit ELISA, Svp4 Kit ELISA, ACDC Kit ELISA, ACRP30 Kit ELISA, ADIPQTL1 Kit ELISA, ADPN Kit ELISA, APM-1 Kit ELISA, APM1 Kit ELISA, GBP28 Kit ELISA, apM-1 Kit ELISA, 30kDa Kit ELISA, APN Kit ELISA, Acdc Kit ELISA, Acrp30 Kit ELISA, Ad Kit ELISA, adipo Kit ELISA, apM1 Kit ELISA, ADN Kit ELISA, acrpl Kit ELISA, adipo-b Kit ELISA, adipoql Kit ELISA, zgc:153584 Kit ELISA, adipoq Kit ELISA, WD and tetratricopeptide repeats 1 Kit ELISA, MAM domain containing glycosylphosphatidylinositol anchor 2 Kit ELISA, seminal vesicle secretory protein 4 Kit ELISA, adiponectin, C1Q and collagen domain containing Kit ELISA, adiponectin, C1Q and collagen domain containing, b Kit ELISA, adiponectin, C1Q and collagen domain containing L homeolog Kit ELISA, WDTC1 Kit ELISA, Mdga2 Kit ELISA, Svs4 Kit ELISA, ADIPOQ Kit ELISA, Adipoq Kit ELISA, adipoqb Kit ELISA, adipoq.L Kit ELISA

Sujet:

Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Synonyms: 30 kDa adipocyte complement-related protein ,Adiponectin, C1Q and collagen domain containing ,Protein Adipoq ,Adipoq ,Acdc ,rCG_36676 ,

Full Gene Name: adiponectin, C1Q and collagen domain containing

ID gène: 246253

UniProt: Q8K3R4