Global DNA Methylation - LINE-1 Kit

N° du produit ABIN2866120

Aperçu rapide pour Global DNA Methylation - LINE-1 Kit (ABIN2866120)

Détail du produit

Specificité: The Global DNA Methylation - LINE-1 Kit is tested for sensitivity in detecting changes in global DNA methylation levels in 100 ng genomic DNA extracted from HeLa cells that are untreated or treated with 0.05 μM 5-azacytidine, a known inhibitor of DNA methyltransferases (DNMTs).

Information d'application

Commentaires:

The DNA is MseI digested and hybridized to the provided LINE-1 probe according to the product manual instructions for capture of the Long Interspersed Nucleotide Element 1 repeat elements of human genomic DNA. The provided methylated and non-methylated DNA standards are used to generate a standard curve for DNA quantification. The OD 450nm results of Lot 11115003 with a 2.5 minute developing time are shown in Figure 1. The data is then blanked and the net readings are graphed using a quadratic equation to generate a standard curve for quantification (Figure 2). Results show a reduction in global DNA methylation for the 5-azacytidine-treated HeLa cells as expected.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Components can be stored at -20°C prior to first use, then we recommend storing the components at the temperatures listed in the manual.