Estrogen Receptor alpha Kit ELISA

N° du produit ABIN2866264

Détail du produit Estrogen Receptor alpha Kit ELISA

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marque: TransAM®

Type d'échantillon: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificité: TransAM ER Kits are tested for sensitivity in detecting ER in nuclear extracts from MCF-7 cells that are either untreated or post-treated with H₂O₂.

Attributs du produit: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Ingrédients: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

These extracts are diluted down to 0.313 μg/well and assayed using the TransAM ER Kit. The ratio of the signals from the treated over the untreated cells must be above 4. Lot No. 01403001 was developed for 5 minutes. It gave a ratio of 17 (Figure 1). The basal level of ER expression, and this ratio, may vary depending on the cell type tested and the treatment used. TransAM ER Kits are also tested for specificity in detecting ER activation. TransAM ER assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated ER consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on ER binding.

Durée du test: 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the primary antibody, oligonucleotides, DTT, Poly [d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.

Date de péremption: 6 months

Détail du antigène

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: Estrogen Receptor 1 (ESR1 Produits)

Synonymes: LOC398734 Kit ELISA, AA420328 Kit ELISA, AU041214 Kit ELISA, ER-alpha Kit ELISA, ER[a] Kit ELISA, ERa Kit ELISA, ERalpha Kit ELISA, ESR Kit ELISA, Estr Kit ELISA, Estra Kit ELISA, Nr3a1 Kit ELISA, NR3A1 Kit ELISA, eralpha Kit ELISA, zfER[a] Kit ELISA, ER Kit ELISA, ESRA Kit ELISA, ESTRR Kit ELISA, Era Kit ELISA, akap12 Kit ELISA, cb27 Kit ELISA, esr1 Kit ELISA, fb72g12 Kit ELISA, id:ibd1202 Kit ELISA, sb:cb27 Kit ELISA, si:ch73-192g21.1 Kit ELISA, wu:fb72g12 Kit ELISA, wu:fc21c03 Kit ELISA, Esr Kit ELISA, RNESTROR Kit ELISA, ERALPHA Kit ELISA, er Kit ELISA, esr Kit ELISA, nr3a1 Kit ELISA, XER Kit ELISA, era Kit ELISA, esra Kit ELISA, ERbeta Kit ELISA, xesr-1 Kit ELISA, xlERalpha1 Kit ELISA, xlERalpha2 Kit ELISA, ESR1 Kit ELISA, estrogen receptor 1 L homeolog Kit ELISA, estrogen receptor 1 (alpha) Kit ELISA, estrogen receptor 1 Kit ELISA, A kinase (PRKA) anchor protein 12b Kit ELISA, esr1.L Kit ELISA, Esr1 Kit ELISA, ESR1 Kit ELISA, esr1 Kit ELISA, akap12b Kit ELISA

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly