ACTH Kit FLISA

N° du produit ABIN2871073

Ce kit ELISA Fluorometric est conçu pour la mesure quantitative de Souris et Rat ACTH.

Aperçu rapide pour ACTH Kit FLISA (ABIN2871073)

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivité: Souris, Rat

Méthode de détection: Fluorometric

Type de méthode: Competition ELISA

Application: ELISA

Détail du produit ACTH Kit FLISA

Analytical Method: Quantitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: ACTH

Classe de substances: Hormone

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity