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Urocortin Kit FLISA

UCN Reactivité: Humain Fluorometric Competition ELISA
N° du produit ABIN2871245
  • Antigène Voir toutes Urocortin (UCN) Kits FLISA
    Urocortin (UCN)
    Reactivité
    • 3
    • 3
    • 2
    Humain
    Méthode de détection
    Fluorometric
    Type de méthode
    Competition ELISA
    Application
    ELISA
    Analytical Method
    Quantitative
    Top Product
    Discover our top product UCN Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    Restrictions
    For Research Use only
  • Stock
    4 °C
  • Antigène Voir toutes Urocortin (UCN) Kits FLISA
    Urocortin (UCN)
    Autre désignation
    Urocortin (UCN Produits)
    Synonymes
    UCN Kit FLISA, si:ch211-243j20.4 Kit FLISA, ucn Kit FLISA, wu:fq17a01 Kit FLISA, UI Kit FLISA, UROC Kit FLISA, Mpv17 Kit FLISA, Ucn1 Kit FLISA, urocortin Kit FLISA, urotensin 1 Kit FLISA, UCN Kit FLISA, uts1 Kit FLISA, Ucn Kit FLISA
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of Cell Size, Feeding Behaviour
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