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Exendin 4 Kit FLISA

Reactivité: Humain Fluorometric Competition ELISA
N° du produit ABIN2871401
  • Antigène Voir toutes Exendin 4 Kits FLISA
    Exendin 4
    Reactivité
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Fluorometric
    Type de méthode
    Competition ELISA
    Application
    ELISA
    Analytical Method
    Quantitative
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    Restrictions
    For Research Use only
  • Stock
    4 °C
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    Exendin 4
    Autre désignation
    Exendin-4 (Exendin 4 Produits)
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