Corticosterone Kit ELISA

N° du produit ABIN2946686

Kit ELISA Différentes espèces Corticosterone, test Colorimetric pour la quantification de Différentes espèces Corticosterone.

Aperçu rapide pour Corticosterone Kit ELISA (ABIN2946686)

Antigène: Corticosterone (CORT)

Reactivité: Différentes espèces

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.39 ng/mL - 25 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Corticosterone Kit ELISA

Seuil minimal de détection: 0.39 ng/mL

Fonction: Corticosterone (17-Deoxycortisol) ELISA Kit

Analytical Method: Quantitative

Sensibilité: 0.24 ng/mL

Ingrédients: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Matériel non inclus:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Information d'application

Indications d'application: Optimal dilutions/concentrations should be determined by the end user.

Commentaires:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plaque: Pre-coated

Préparation des réactifs:

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.

• 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.

• 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

Prélèvement de l'échantillon:

• Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4 °C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 x g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C or -80 °C.

• Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay immediately or aliquot and store at -20 °C or -80 °C. Avoid hemolysis and high cholesterol samples.

• Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type - this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 x g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20 °C.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Corticosterone (17-Deoxycortisol) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Corticosterone (17-Deoxycortisol) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Date de péremption: 6 months

Détail du antigène

Antigène: Corticosterone (CORT)

Autre désignation: Corticosterone

Classe de substances: Hormone