LC3B Kit ELISA

N° du produit ABIN2949507

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain LC3B.

Aperçu rapide pour LC3B Kit ELISA (ABIN2949507)

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit LC3B Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Human Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit

Analytical Method: Quantitative

Sensibilité: 18.8 pg/mL

Ingrédients: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Matériel non inclus:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Information d'application

Indications d'application: Optimal dilutions/concentrations should be determined by the end user.

Commentaires:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plaque: Pre-coated

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Microtubule-associated proteins 1A/1B light chain 3B were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Microtubule-associated proteins 1A/1B light chain 3B were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Date de péremption: 6 months

Détail du antigène

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Autre désignation: Microtubule-Associated Proteins 1A/1B Light Chain 3B

Sujet: MLP3B_HUMAN

ID gène: 81631

HGNC: 13352

OMIM: 609604

UniProt: Q9GZQ8

Pathways: Autophagy