TPSAB1 Kit ELISA

N° du produit ABIN3044697

Kit ELISA Humain TPSAB1, test Colorimetric pour la quantification de Humain TPSAB1.

Aperçu rapide pour TPSAB1 Kit ELISA (ABIN3044697)

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Épitope: AA 1-275

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit TPSAB1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Tryptase/TPSAB1,B2

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: M1-V275

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <15pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Immunogen sequence: M1-V275

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin, in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Plaque: Pre-coated

Protocole: human Tryptase ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tryptase has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: M1-V275) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tryptase is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tryptase amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Tryptase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Tryptase standard solution and each sample be measured in duplicate.

Restrictions: For Research Use only

Stockage

Buffer: heparin or EDTA

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Autre désignation: TPSAB1/TPSB2

Sujet:

Protein Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .

Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Synonyms: Tryptase beta-2,Tryptase-2,3.4.21.59,Tryptase II,TPSB2,TPS2,

Full Gene Name: Tryptase beta-2

Cellular Localisation: Secreted. Released from the secretory granules upon mast cell activation.

ID gène: 7177, 64499

UniProt: P20231