Aperçu rapide pour Nitrite Determination Assay Kit (ABIN7384583)
Méthode de détection
Application
Type d'échantillon
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Gamme de detection
- 1-100 μM
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Seuil minimal de détection
- 1 μM
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Fonction
- The kit is designed for nitrite determination in biological samples.
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Specificité
- Specific for nitrite determination in plasma samples.
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Attributs du produit
- The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
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Ingrédients
- Plate and the reagents neccesary to perform the assay.
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Matériel non inclus
- Pipettes, reaction tubes, plate reader.
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Volume d'échantillon
- 50 μL
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Durée du test
- 1 h
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Plaque
- Uncoated
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Préparation des réactifs
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No reagent preparation is needed.
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Préparation de l'échantillon
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Plasma samples may be deproteinized before performing the assay.
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Procédure de l'essai
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Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 ( see in booklet) . Use ultrapure water as diluent. Performing the assay: 1. Add 50 μL of samples or standards in each well (96-well plate). 2.Add 50 μL of Reagent A in each well. Incubate for 10 minutes protected from light. 3.Add 50 μL of Reagent B in each well. Incubate for 10 minutes protected from light. 4.Read the absorbance at 540 nm within 30 minutes.
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Calcul des résultats
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Determine average absorbance value of each experimental sample. Determine its concentration by comparison to the Nitrite Standard reference curve (Figure 2 see images). Nitrite (μM) = (?A540nm- intercept)/ slope
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Restrictions
- For Research Use only
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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