Fibrinogen Kit ELISA

N° du produit ABIN365101

Kit ELISA Fibrinogen Humain, Colorimetric test pour la quantification de Humain Fibrinogen et a été mentionné dans 2+ publications.

Aperçu rapide pour Fibrinogen Kit ELISA (ABIN365101)

Antigène: Fibrinogen

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 μg/mL - 20 μg/mL

Application: ELISA

Type d'échantillon: Serum, Plasma, Tissue Homogenate

Détail du produit Fibrinogen Kit ELISA

Seuil minimal de détection: 0.312 μg/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of human Fbg concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human Fbg.

Réactivité croisée (Details): Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Sensibilité: 0.078 μg/mL

Ingrédients:

• Assay plate (12 × 8 coated Microwells)
• Standard (freeze dried)
• Biotin-antibody (100 × concentrate)
• HRP-avidin (100 × concentrate)
• Biotin-antibody Diluent
• HRP-avidin Diluent
• Sample Diluent
• Wash Buffer (25 × concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip (for 96 wells)
• Instruction manual

Information d'application

Indications d'application:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
• Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
• Grossly hemolyzed samples are not suitable for use in this assay.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Commentaires:

Detection wavelength: 450 nm

Information on standard material:
Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fbg. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Fbg and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Fbg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Fbg in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.

• Intra-assay: CV% less than 8%
• Inter-assay: CV% less than 10%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Conseil sur la manipulation:

• The kit should not be used beyond the expiration date on the kit label.
• Do not mix or substitute reagents with those from other lots or sources.
• If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
• Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
• This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Stock: 4 °C/-20 °C

Stockage commentaire: For unopened kit: All the reagents should be kept according to the labels on vials.

Date de péremption: 6 months

Références pour Fibrinogen Kit ELISA (ABIN365101)

Islam, Huang, Fanelli, Delsedime, Wu, Khang, Han, Grassi, Li, Xu, Luo, Wu, Liu, McKillop, Medin, Qiu, Zhong, Liu, Laffey, Li, Zhang: "Identification and Modulation of Microenvironment is Crucial for Effective MSC Therapy in Acute Lung Injury." dans: American journal of respiratory and critical care medicine, (2018) (PubMed).

Bin, Liang, Ou, Cui, Luo: "Two symptomatic cases of dysfibrinogenemia in China: one with gamma-chain Arg275Cys mutation and another without detectable mutation in fibrinogen genes." dans: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, Vol. 26, Issue 5, pp. 564-71, (2015) (PubMed).

Détail du antigène

Antigène: Fibrinogen

Autre désignation: Fibrinogen (Fbg)

Sujet: Fibrinogen1 circulates in the plasma at a concentration of 2 g/L to 4 g/L with a half-life of approximately 4 days and is a modest acute-phase reactant, increasing in concentration in response to most forms of tissue injury, infection, or inflammation. The native 340-kDa molecule is a disulfide-bonded homodimer, each protomer composed of 3 nonidentical disulfide cross-linked polypeptide chains that are synthesized under the control of 3 genes. Fibrinogen is produced by cultured hepatocytes in vitro, and plasma fibrinogen has been demonstrated in vivo to come from the liver in rats. However, in response to inflammatory mediators, expression of mRNA for individual polypeptide chains and their synthesis and/or secretion in vitro has also been described in a variety of nonhepatic cells, including epithelial cells from various different human tissues, granulosa cells, cervical carcinoma cells, and trophoblasts. Extra-hepatic fibrinogen synthesis may be important for tissue repair at local sites of injury, and/or may have a pathogenetic role, but it is not known whether it contributes significantly to either the normal plasma fibrinogen concentration or to increased concentrations in the acute-phase response..