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PPIA Kit ELISA

PPIA Reactivité: Souris Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
N° du produit ABIN366417
  • Antigène Voir toutes PPIA Kits ELISA
    PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
    Reactivité
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    For the quantitative determination of mouse cyclophilin A (CyPA) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
    Type d'échantillon
    Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of mouse CyPA.
    Réactivité croisée (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensibilité
    3.9 pg/mL
    Ingrédients
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Indications d'application
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Commentaires

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CyPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CyPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CyPA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CyPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Préparation des réactifs
    • Biotin-antibody (1×) - Centrifuge the vial before opening.
      Biotin-antibody requires a 100-fold dilution. The suggested dilution is 10µL of Biotin-antibody + 990µL of Biotin-antibody Diluent.
    • HRP-avidin (1×) - Centrifuge the vial before opening.
      HRP-avidin requires a 100-fold dilution. The suggested dilution is 10µL of HRP-avidin + 990µL of HRP-avidin Diluent.
    • Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20mL of Wash Buffer Concentrate (25×) into deionized or distilled water to prepare 500mL of Wash Buffer (1×).
    • Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
      Reconstitute the Standard with 1ml of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
      Pipette 250µL of Sample Diluent into each tube. Use the stock solution to produce a 2-fold dilution series. Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard (0ng/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25°C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction. Avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL when pipetting.
    • It is recommended to use distilled water to prepare reagents and samples. Using contaminated water or container for reagent preparation will influence detection result.
    Précision du teste
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Conseil sur la manipulation
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Date de péremption
    6 months
  • Song, Yang, Chen, Huang, Yin, He, Huang, Hu: "Prognosis of sepsis induced by cecal ligation and puncture in mice improved by anti-Clonorchis Sinensis cyclopholin a antibodies." dans: Parasites & vectors, Vol. 8, pp. 502, (2015) (PubMed).

  • Antigène Voir toutes PPIA Kits ELISA
    PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
    Autre désignation
    Cyclophilin A (CyPA) (PPIA Produits)
    Synonymes
    Cyp1 Kit ELISA, PPIA-2 Kit ELISA, fa93g09 Kit ELISA, ppia Kit ELISA, wu:fa93g09 Kit ELISA, wu:fb05e11 Kit ELISA, wu:fb13h02 Kit ELISA, wu:fb15a05 Kit ELISA, CYPA Kit ELISA, CypA Kit ELISA, Cypa Kit ELISA, CYPH Kit ELISA, CYCA Kit ELISA, CyP-A Kit ELISA, MGC75715 Kit ELISA, 2700098C05 Kit ELISA, Cphn Kit ELISA, CyP-18 Kit ELISA, MT-ND1 Kit ELISA, MTND1 Kit ELISA, NADH1 Kit ELISA, ND1 Kit ELISA, cypA Kit ELISA, PPIase A Kit ELISA, ppial Kit ELISA, wu:fj18g05 Kit ELISA, zgc:73102 Kit ELISA, zgc:86688 Kit ELISA, peptidylprolyl isomerase A Kit ELISA, peptidylprolyl isomerase Aa (cyclophilin A) Kit ELISA, peptidylprolyl isomerase A (cyclophilin A) L homeolog Kit ELISA, cyclophilin A Kit ELISA, cyclophilin a Kit ELISA, peptidylprolyl isomerase A (cyclophilin A) Kit ELISA, peptidylprolyl isomerase Ab (cyclophilin A) Kit ELISA, PPIA Kit ELISA, ppiaa Kit ELISA, ppia.L Kit ELISA, Cypa Kit ELISA, CNB01230 Kit ELISA, CNB01290 Kit ELISA, Tc00.1047053506925.300 Kit ELISA, Tb11.03.0250 Kit ELISA, CYPA Kit ELISA, Ppia Kit ELISA, ppia Kit ELISA, ppiab Kit ELISA
    Sujet
    Synonyms: CYPA, CYPH, MGC117158, MGC12404, MGC23397, PPIase A|T cell cyclophilin|cyclosporin A-binding protein|peptidyl-prolyl cis-trans isomerase A|peptidylprolyl isomerase A|rotamase A
    HGNC
    9253
    UniProt
    P17742
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