CX3CL1 Kit ELISA

N° du produit ABIN411274

Kit ELISA Souris CX3CL1, test Colorimetric pour la quantification de Souris CX3CL1.

Aperçu rapide pour CX3CL1 Kit ELISA (ABIN411274)

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Épitope: AA 25-337

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Détail du produit CX3CL1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Fractalkine/CX3CL1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21
Immunogen sequence: Q25-R337

Réactivité croisée (Details): There is no detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: Q25-R337

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Fractalkine has been precoated onto 96-well plates. Standards(sf21, Q25-R337) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Fractalkine amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Fractalkine standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin, EDTA) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Fractalkine standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.57, Standard deviation: 0.113, CV(%): 7.2
• Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.224, CV(%): 6.4
• Sample 3: n=16, Mean(ng/ml): 6.3, Standard deviation: 0.473, CV(%): 7.5,
• Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.11, CV(%): 8.3
• Sample 2: n=24, Mean(ng/ml): 3.7, Standard deviation: 0.285, CV(%): 7.7
• Sample 3: n=24, Mean(ng/ml): 6.4, Standard deviation: 0.544, CV(%): 8.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Autre désignation: CX3CL1

Sujet:

Background: Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

Synonyms: Chemokine (C-X3-C motif) ligand 1 ,cDNA, FLJ94424, Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), mRNA ,CX3CL1 ,hCG_15105 ,

Full Gene Name: chemokine (C-X3-C motif) ligand 1

ID gène: 6376

UniProt: A0N0N7

Pathways: Synaptic Membrane