ICAM1 Kit ELISA

N° du produit ABIN411280

Kit ELISA ICAM1 Souris, Colorimetric test pour la quantification de Souris ICAM1 et a été mentionné dans 14+ publications.

Aperçu rapide pour ICAM1 Kit ELISA (ABIN411280)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Épitope: AA 28-485

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit ICAM1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Mouse ICAM-1 / CD54 ELISA Kit to quantitate Mouse Icam1 in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: Q28-N485

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6.2%, 4.5%, 6.4%
Inter-Assay Precision (CV%): 7%, 5.7%, 8%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour ICAM1 Kit ELISA (ABIN411280)

Zhang, Mu, Zhou, Song, Zhang, Wu, Jiang, Wang, Zhang, Li, Shao, Wang, Li, Yang, Wu, Zhang, Tang: "Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction." dans: PLoS ONE, Vol. 11, Issue 6, pp. e0158038, (2017) (PubMed).

Xu, Li, Dong, Tan, Wu, Xie, Yang: "Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE-/- Mice through the ROS/MAPK/NF-κB Pathway." dans: Nutrients, Vol. 7, Issue 8, pp. 7085-105, (2016) (PubMed).

Barros, Borges, Ferreira, Carmo, Rosado, Fouque, Mafra: "Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?" dans: Future microbiology, Vol. 10, Issue 4, pp. 517-26, (2015) (PubMed).

Xin, Sun, Kong, Li, Zheng, Yin, Cao, Jia, Li: "Intercellular adhesion molecule-1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps." dans: Molecular medicine reports, Vol. 11, Issue 5, pp. 3391-7, (2015) (PubMed).

Tian, Wang, Cao, Zhong, Tu, Xiao, He, Zhai: "Comparison of protective effects of safflor injection and extract of Ginkgo biloba on lung ischemia/reperfusion injury in rabbits." dans: Chinese journal of integrative medicine, Vol. 21, Issue 3, pp. 229-33, (2015) (PubMed).

Jiang, Zhang, Liu, Lin, Sun, Shen, Yan, Xie: "EETs alleviate ox-LDL-induced inflammation by inhibiting LOX-1 receptor expression in rat pulmonary arterial endothelial cells." dans: European journal of pharmacology, Vol. 727, pp. 43-51, (2014) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." dans: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Jing, Wang, Zhang, Cao, Chang, Dong, Guo, Li: "Upregulation of ICAM-1 in diabetic rats after transient forebrain ischemia and reperfusion injury." dans: Journal of inflammation (London, England), Vol. 11, Issue 1, pp. 35, (2014) (PubMed).

Asgary, Kelishadi, Rafieian-Kopaei, Najafi, Najafi, Sahebkar: "Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents." dans: Pediatric cardiology, Vol. 34, Issue 7, pp. 1729-35, (2013) (PubMed).

Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." dans: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." dans: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

Xiang, Qian, Zhou, Liu, Li: "Crocetin inhibits leukocyte adherence to vascular endothelial cells induced by AGEs." dans: Journal of ethnopharmacology, Vol. 107, Issue 1, pp. 25-31, (2006) (PubMed).

Yang, Rui, Lu, Li, Liu, Yan, Wang: "Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats." dans: Life sciences, Vol. 77, Issue 20, pp. 2529-39, (2005) (PubMed).

Boivin, Chavassieux, Chapuy, Baud, Meunier: "Skeletal fluorosis: histomorphometric analysis of bone changes and bone fluoride content in 29 patients." dans: Bone, Vol. 10, Issue 2, pp. 89-99, (1989) (PubMed).

Détail du antigène

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM1

Classe de substances: Viral Protein

Sujet:

Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8 kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

Gene Full Name: intercellular adhesion molecule 1

ID gène: 15894

UniProt: P13597

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling