CCL22 Kit ELISA

N° du produit ABIN411323

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain CCL22. Il a été cité dans 2 publications.

Aperçu rapide pour CCL22 Kit ELISA (ABIN411323)

Antigène: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Épitope: AA 25-93

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit CCL22 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Human CCL22/MDC ELISA Kit to quantitate Human CCL22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: G25-Q93

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.6%, 4.3%, 6.6%
Inter-Assay Precision (CV%): 9.1%, 6%, 7.4%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour CCL22 Kit ELISA (ABIN411323)

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." dans: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Chen, Hu, Mao, Jiao, Wang, Yu, Xu, Dai, Yin, Xu: "Increased IL-17-producing CD4(+) T cells in patients with esophageal cancer." dans: Cellular immunology, Vol. 272, Issue 2, pp. 166-74, (2011) (PubMed).

Détail du antigène

Antigène: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Autre désignation: CCL22

Sujet:

Background: Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37 % identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35 % identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Gene Full Name: C-C motif chemokine ligand 22

ID gène: 6367

UniProt: O00626