MMP2 Kit ELISA

N° du produit ABIN411329

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris MMP2. Il a été cité dans 19 publications.

Aperçu rapide pour MMP2 Kit ELISA (ABIN411329)

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Épitope: AA 30-662

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit MMP2 Kit ELISA

Seuil minimal de détection: 625 pg/mL

Fonction: Sandwich Mouse MMP-2 ELISA Kit to quantitate Mouse Mmp2 in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: A30-C662

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.5%, 4.6%, 4.4%
Inter-Assay Precision (CV%): 5.6%, 4.7%, 4.6%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour MMP2 Kit ELISA (ABIN411329)

Xie, Huo, Li, Dai, Xu, Yin: "Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway." dans: Cell transplantation, Vol. 26, Issue 6, pp. 967-982, (2018) (PubMed).

Chen, Zeng, Zhan, Wang, Jiang, Li: "Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b." dans: Oncogene, Vol. 36, Issue 33, pp. 4692-4705, (2017) (PubMed).

Bai, Yin, Feng, Cao, Wu, Zhu, Li, Tu, Chai: "Corydalis hendersonii Hemsl. protects against myocardial injury by attenuating inflammation and fibrosis via NF-κB and JAK2-STAT3 signaling pathways." dans: Journal of ethnopharmacology, Vol. 207, pp. 174-183, (2017) (PubMed).

Dai, Ji, Jiang, Sun: "Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma." dans: Oncology letters, Vol. 13, Issue 5, pp. 2897-2902, (2017) (PubMed).

Frati, Ricci, Pierucci, Nistri, Bani, Meacci: "Role of sphingosine kinase/S1P axis in ECM remodeling of cardiac cells elicited by relaxin." dans: Molecular endocrinology (Baltimore, Md.), Vol. 29, Issue 1, pp. 53-67, (2016) (PubMed).

Papakonstantinou, Karakiulakis, Batzios, Savic, Roth, Tamm, Stolz: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." dans: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).

Aragon, Erdely, Bishop, Salmen, Weaver, Liu, Hall, Eye, Kodali, Zeidler-Erdely, Stafflinger, Ottens, Campen: "MMP-9-Dependent Serum-Borne Bioactivity Caused by Multiwalled Carbon Nanotube Exposure Induces Vascular Dysfunction via the CD36 Scavenger Receptor." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 150, Issue 2, pp. 488-98, (2016) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." dans: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." dans: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." dans: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." dans: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

Qin, Liu, Li, Liu, Li, Gao, Shao, Zhen: "MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine." dans: Oncology reports, Vol. 32, Issue 1, pp. 121-30, (2014) (PubMed).

Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." dans: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." dans: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Tang, Tang, Fang, Liang, Zhang: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." dans: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." dans: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." dans: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." dans: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." dans: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Détail du antigène

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Autre désignation: MMP2

Sujet:

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Gene Full Name: matrix metallopeptidase 2

ID gène: 17390

UniProt: P33434

Pathways: Activation of Innate immune Response