MMP3 Kit ELISA

N° du produit ABIN411330

Le kit ELISA MMP3 (ABIN411330) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain MMP3. Ce kit ELISA a été cité dans 10 publications.

Aperçu rapide pour MMP3 Kit ELISA (ABIN411330)

Antigène: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Épitope: AA 18-477

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit MMP3 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Human MMP-3 ELISA Kit to quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: Y18-C477

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is cross-reactivity with MMP-10 approximately 2 % , and no detectable cross-reactivity with other MMPs.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6.9%, 6.5%, 6.4%
Inter-Assay Precision (CV%): 6.2%, 6.8%, 6.9%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour MMP3 Kit ELISA (ABIN411330)

Lin, Qiu, Xie, Liu, Sun: "Nimbolide suppresses non-small cell lung cancer cell invasion and migration via manipulation of DUSP4 expression and ERK1/2 signaling." dans: Biomedicine & pharmacotherapy, Vol. 92, pp. 340-346, (2017) (PubMed).

Nosratzehi, Alijani, Moodi: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" dans: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).

Ozcamdalli, Misir, Kizkapan, Uzun, Duygulu, Yazici, Kafadar: "Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis: A Pilot Study." dans: Cartilage, Vol. 8, Issue 4, pp. 384-390, (2017) (PubMed).

Qiu, Li, Zhang, Liu, Tian, Fang: "P2X7 mediates ATP-driven invasiveness in prostate cancer cells." dans: PLoS ONE, Vol. 9, Issue 12, pp. e114371, (2015) (PubMed).

Capsoni, Ongari, Lonati, Accetta, Gatti, Catania: "?-Melanocyte-stimulating-hormone (?-MSH) modulates human chondrocyte activation induced by proinflammatory cytokines." dans: BMC musculoskeletal disorders, Vol. 16, pp. 154, (2015) (PubMed).

Zhu, Liu, Li, Zhang: "Eotaxin-1 promotes prostate cancer cell invasion via activation of the CCR3-ERK pathway and upregulation of MMP-3 expression." dans: Oncology reports, Vol. 31, Issue 5, pp. 2049-54, (2014) (PubMed).

Ågren, Schnabel, Christensen, Mirastschijski: "Tumor necrosis factor-?-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo." dans: European journal of cell biology, Vol. 94, Issue 1, pp. 12-21, (2014) (PubMed).

Bellei, Pitisci, Ottaviani, Ludovici, Cota, Luzi, DellAnna, Picardo: "Vitiligo: a possible model of degenerative diseases." dans: PLoS ONE, Vol. 8, Issue 3, pp. e59782, (2014) (PubMed).

Zhao, Fan, Zhang, Sun, Li, Xiong, Zhang, Fan: "Chitosan-plasmid DNA nanoparticles encoding small hairpin RNA targeting MMP-3 and -13 to inhibit the expression of dedifferentiation related genes in expanded chondrocytes." dans: Journal of biomedical materials research. Part A, Vol. 102, Issue 2, pp. 373-80, (2013) (PubMed).

An, Wu, Lin: "Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts." dans: International journal of ophthalmology, Vol. 4, Issue 4, pp. 353-6, (2012) (PubMed).

Détail du antigène

Antigène: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Autre désignation: MMP3

Sujet:

Background: Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54 % identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.

Gene Full Name: matrix metallopeptidase 3

ID gène: 4314

UniProt: P08254