Nerve Growth Factor Kit ELISA

N° du produit ABIN411335

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Nerve Growth Factor. Il a été cité dans 7 publications.

Aperçu rapide pour Nerve Growth Factor Kit ELISA (ABIN411335)

Antigène: Nerve Growth Factor (NGF)

Épitope: AA 122-241

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit Nerve Growth Factor Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit to quantitate Human NGF in cell culture supernatants and serum.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: S122-A241

Capture antibody and Detection antibody: polyclonal antibody from rabbit, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 15 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 2.1%, 5.7%, 6%
Inter-Assay Precision (CV%): 6.2%, 7.7%, 6.7%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour Nerve Growth Factor Kit ELISA (ABIN411335)

Zhang, Wang, Zhang, Zhao: "Improvement of postoperative cognitive dysfunction and attention network function of patients with ischemic cerebrovascular disease via dexmedetomidine." dans: Experimental and therapeutic medicine, Vol. 15, Issue 3, pp. 2968-2972, (2018) (PubMed).

Gamper, Viereck: "Re: Sheng W, Zhang H, Kirschner-Hermanns R. Could urinary nerve growth factor be a biomarker for overactive bladder? A meta-analysis. Neurourol Urodyn. 2017;9999:1-8. doi: 10.1002/nau.23210." dans: Neurourology and urodynamics, (2017) (PubMed).

Ogura, Suzuki, Sakuma, Yamauchi, Orita, Miyagi, Ishikawa, Kamoda, Oikawa, Kanisawa, Takahashi, Sakai, Nagamine, Fukuda, Takahashi, Ohtori, Tsuchiya: "Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions." dans: Journal of experimental orthopaedics, Vol. 3, Issue 1, pp. 7, (2016) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." dans: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Zakharyan, Atshemyan, Gevorgyan, Boyajyan: "Nerve growth factor and its receptor in schizophrenia." dans: BBA clinical, Vol. 1, pp. 24-9, (2015) (PubMed).

Forsyth, Krishna, Lawn, Valadez, Qu, Fenstermacher, Fournier, Potthast, Chinnaiyan, Gibney, Zeinieh, Barker, Carter, Cooper, Kenchappa: "p75 neurotrophin receptor cleavage by ?- and ?-secretases is required for neurotrophin-mediated proliferation of brain tumor-initiating cells." dans: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8067-85, (2014) (PubMed).

Tan, Xie, Liu, Chen, Zheng, Tong, Tian: "Low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) reverses A?(1-42)-mediated memory deficits in rats." dans: Experimental gerontology, Vol. 48, Issue 8, pp. 786-94, (2013) (PubMed).

Détail du antigène

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF

Sujet:

Background: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Gene Full Name: nerve growth factor

ID gène: 4803

UniProt: P01138

Pathways: Regulation of Cell Size