Osteopontin Kit ELISA

N° du produit ABIN411343

Kit ELISA Osteopontin Humain, Colorimetric test pour la quantification de Humain Osteopontin et a été mentionné dans 4+ publications.

Aperçu rapide pour Osteopontin Kit ELISA (ABIN411343)

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Épitope: AA 17-300

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk, Urine

Détail du produit Osteopontin Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Human OPN / Osteopontin ELISA Kit to quantitate Human SPP1 in cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and urine.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: I17-N300

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.6%, 7.8%, 6.1%
Inter-Assay Precision (CV%): 5.3%, 8.3%, 7.1%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour Osteopontin Kit ELISA (ABIN411343)

Qin, Yan, Wang, Xu, Wang, Zhu, Shi, Li, Zhang, Chen: "Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway." dans: Theranostics, Vol. 8, Issue 4, pp. 921-940, (2018) (PubMed).

El-Din, Amin, Egiza: "Utility of Tissue Inhibitor Metalloproteinase-1 and Osteopontin as Prospective Biomarkers of Early Cardiovascular Complications in Type 2 Diabetes." dans: Open access Macedonian journal of medical sciences, Vol. 6, Issue 2, pp. 314-319, (2018) (PubMed).

Sun, Tu, Chen, Yuan, Liu, Dong, Yuan: "Three-dimensional combined biomarkers assay could improve diagnostic accuracy for gastric cancer." dans: Scientific reports, Vol. 7, Issue 1, pp. 11621, (2017) (PubMed).

Chen, Sun, He, Gong, Xu, Yuan: "Serum OPN expression for identification of gastric cancer and atrophic gastritis and its influencing factors." dans: PLoS ONE, Vol. 9, Issue 12, pp. e114005, (2014) (PubMed).

Détail du antigène

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Autre désignation: SPP1

Sujet:

Background: Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Gene Full Name: secreted phosphoprotein 1

ID gène: 6696

UniProt: P10451

Pathways: Regulation of Cell Size