PDGF-AB Heterodimer Kit ELISA

N° du produit ABIN411346

Détail du produit

Antigène: PDGF-AB Heterodimer

Épitope: AA 74-182, AA 86-204

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat PDGF-AB

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: (R86-K204)+(S74-T182)

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <3pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: (R86-K204)+(S74-T182)

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: rat PDGF-AB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-AB has been precoated onto 96-well plates. Standards(E.coli, (R86-K204)+(S74-T182)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat PDGF-AB amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat PDGF-AB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat PDGF-AB standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 246, Standard deviation: 14.27, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 632, Standard deviation: 34.76, CV(%): 5.5
• Sample 3: n=16, Mean(pg/ml): 1068, Standard deviation: 50.2, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 275, Standard deviation: 20.35, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 723, Standard deviation: 45.55, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 1249, Standard deviation: 73.7, CV(%): 5.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: PDGF-AB Heterodimer

Autre désignation: Platelet-derived growth factor (PDGF) (PDGF-AB)

Synonymes: PDGF-A Kit ELISA, PDGF1 Kit ELISA, platelet derived growth factor subunit A Kit ELISA, PDGFA Kit ELISA

Sujet:

Protein Function: Pdgfa: Growth factor that plays an essential role in theregulation of embryonic development, cell proliferation, cellmigration, survival and chemotaxis. Potent mitogen for cells ofmesenchymal origin. Required for normal lung alveolar septumformation during embryogenesis, normal development of thegastrointestinal tract, normal development of Leydig cells andspermatogenesis. Required for normal oligodendrocyte developmentand normal myelination in the spinal cord and cerebellum. Plays animportant role in wound healing. Signaling is modulated by theformation of heterodimers with PDGFB (By similarity).

Background: The platelet-derived growth factor(PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly(96 % ) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus(SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog(PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB(A chain Ser87-Arg196, B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Synonyms: Platelet derived growth factor, B polypeptide ,Platelet-derived growth factor subunit B ,Pdgfb ,rCG_59494 ,

Full Gene Name: platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide

Cellular Localisation: Pdgfa: Secreted. Note: Released by platelets uponwounding.

ID gène: 24628, 25266

UniProt: G3V882