Selectin E/CD62e Kit ELISA

N° du produit ABIN411350

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Selectin E/CD62e. Il a été cité dans 8 publications.

Aperçu rapide pour Selectin E/CD62e Kit ELISA (ABIN411350)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Épitope: AA 22-556

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Détail du produit Selectin E/CD62e Kit ELISA

Seuil minimal de détection: 125 pg/mL

Fonction: Sandwich Human sE-Selectin / CD62E ELISA Kit to quantitate Human SELE in cell culture supernatants, serum and plasma (heparin, citrate).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: W22-P556

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 4 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.9%, 6%, 5.9%
Inter-Assay Precision (CV%): 8%, 7.1%, 7.4%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour Selectin E/CD62e Kit ELISA (ABIN411350)

Dehghan, Sabri, Javanmard, Ahmadi, Mansourian: "Neurally mediated syncope: Is it really an endothelial dysfunction?" dans: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).

Ahluwalia, Misto, Vozzi, Magliaro, Mattei, Marescotti, Avogaro, Iori: "Systemic and vascular inflammation in an in-vitro model of central obesity." dans: PLoS ONE, Vol. 13, Issue 2, pp. e0192824, (2018) (PubMed).

Park, Willoughby, Song, Leutholtz, Koh: "Exercise-induced changes in stress hormones and cell adhesion molecules in obese men." dans: Journal of inflammation research, Vol. 11, pp. 69-75, (2018) (PubMed).

Majidinia, Rasmi, Khadem Ansari, Seyed-Mohammadzad, Saboory, Shirpoor: "Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X." dans: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 3, pp. 561-566, (2016) (PubMed).

Bilgic, Yilmaz, Bozkurt, Celik, Bilgic, Gurel, Kirbas, Bavbek, Akcay: "Relationship of late arteriovenous fistula stenosis with soluble E-selectin and soluble EPCR in chronic hemodialysis patients with arteriovenous fistula." dans: Clinical and experimental nephrology, Vol. 19, Issue 1, pp. 133-9, (2015) (PubMed).

Sabri, Dehghan, Yaghini, Nasiri, Mansourian, Khalifehsoltani: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." dans: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." dans: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." dans: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).

Détail du antigène

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: SELE

Sujet:

Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Gene Full Name: selectin E

ID gène: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling